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mda content detection kit  (Nanjing Jiancheng Bioengineering Research Institute Co Ltd)
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INHBB Facilitates Malignant Progression via Redox Perturbation and EMT Activation. ( A ) Immunoblotting of Bcl-2 and Bax levels following INHBB induction. ( B ) Quantification <t>of</t> <t>SOD</t> activity and <t>MDA</t> content in adherent and suspension states, illustrating altered redox homeostasis. ( C ) Flow cytometric assessment of anoikis tolerance in HT-29 cells. ( D – E ) GO and KEGG enrichment analysis of DEGs modulated by INHBB. ( F ) GSEA plots demonstrating significant enrichment in EMT gene sets. ( G ) Western blot analysis of typical EMT markers following gain- or loss-of-function of INHBB
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INHBB Facilitates Malignant Progression via Redox Perturbation and EMT Activation. ( A ) Immunoblotting of Bcl-2 and Bax levels following INHBB induction. ( B ) Quantification of SOD activity and MDA content in adherent and suspension states, illustrating altered redox homeostasis. ( C ) Flow cytometric assessment of anoikis tolerance in HT-29 cells. ( D – E ) GO and KEGG enrichment analysis of DEGs modulated by INHBB. ( F ) GSEA plots demonstrating significant enrichment in EMT gene sets. ( G ) Western blot analysis of typical EMT markers following gain- or loss-of-function of INHBB

Journal: Biology Direct

Article Title: High INHBB expression in colorectal cancer is associated with poor prognosis and drives malignant phenotypes in tumor cells

doi: 10.1186/s13062-026-00794-4

Figure Lengend Snippet: INHBB Facilitates Malignant Progression via Redox Perturbation and EMT Activation. ( A ) Immunoblotting of Bcl-2 and Bax levels following INHBB induction. ( B ) Quantification of SOD activity and MDA content in adherent and suspension states, illustrating altered redox homeostasis. ( C ) Flow cytometric assessment of anoikis tolerance in HT-29 cells. ( D – E ) GO and KEGG enrichment analysis of DEGs modulated by INHBB. ( F ) GSEA plots demonstrating significant enrichment in EMT gene sets. ( G ) Western blot analysis of typical EMT markers following gain- or loss-of-function of INHBB

Article Snippet: Follow the instructions of the SOD activity detection kit (A001-3-2; Nanjing Jiancheng, China) and the MDA content detection kit (A003-4-1; Nanjing Jiancheng, China), and the experiment was repeated independently 3 times.

Techniques: Activation Assay, Western Blot, Activity Assay, Suspension